Different nutrient media were used to test the totipotency of Canna indica. The Canna responded by exehibiting growth

after the 7-12 days in presence of MS media. It was also observed that Canna grew fast through tissued cultured rasied bulbs

and it was best, when at least 6-8 hours of sunlight each day is provided during the growing season. This protocol for plant

tissue culture raised clone was standardized at National Botanical Research Institute (NBRI), Lucknow laboratories.

Key Words: Canna, Callus regeneration, Field condition, Growth regulators, MS media